High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyze in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase).

HPLC has the ability to separate, and identify compounds that are present in any sample that can be dissolved in a liquid in trace concentrations as low as parts per trillion.

High performance liquid chromatography is now one of the most powerful tools in analytical chemistry. It has the ability to separate, identify, and quantitate the compounds that are presented in any sample that can be dissolved in a liquid.

Because of this versatility, HPLC is used in a variety of industrial and scientific applications, such as pharmaceutical, environmental, forensics, and chemicals.